RNA synthesis in yeast mitochondria by Garrit Jan Boudewijn van Ommen

Cover of: RNA synthesis in yeast mitochondria | Garrit Jan Boudewijn van Ommen

Published by G.J.B. van Ommen in [Amsterdam] .

Written in English

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Subjects:

  • Yeast -- Physiology.,
  • Mitochondria.

Edition Notes

Book details

Other titlesYeast mitochondria.
Statementdoor Garrit Jan Boudewijn van Ommen.
The Physical Object
Pagination143 p. :
Number of Pages143
ID Numbers
Open LibraryOL14211123M

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RNA polymerase activity has been observed in isolated mitochondria of Saccharomyces cerevisiae. Increase in this activity precedes that of other respiratory enzymes, Cited by: In w− strains (not containing this intervening sequence) also a longer form of the 21S rRNA could be observed.

These results suggest that isolated yeast mitochondria are capable of carrying out RNA. A large number of proteins for ATP synthesis and all of the proteins required for mt DNA maintenance, including components of mt DNA replication, transcription, and repair are encoded by the nuclear DNA, and the proteins are synthesized in the cytoplasm and imported into the by: It demonstrates the presence of great progress in the knowledge of structure and functions of the yeast mitochondrial DNA.

This book will be useful to scientific institutes and university laboratories interested in the biochemistry, genetics, and technology of yeasts.

Show less. Biochemistry and Genetics of Yeasts. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. No. 27, Issue of Septem pp. 0 by The American Society for Biochemistry and Molecular Biology, Inc.

Printed in U. S.A. Biosynthesis of tRNA in Yeast Mitochondria. Show less. RNA and Protein Synthesis is a compendium of articles dealing with the assay, characterization, isolation, or purification of various organelles, enzymes, nucleic acids, translational factors, and other components or reactions involved in protein synthesis.

One paper describes the preparatory scale methods for the reversed-phase chromatography systems. To begin studies on the transcription of this genome, we have analyzed RNA synthesis in isolated mitochondria from both wild-type and petite strains of yeast and find that discrete RNA species are Cited by: 7.

In yeast mitochondria, 3′-tRNA processing is an endonucleotic cleavage (25), which is accomplished by a multifunctional RNase Z (11,26,27).

In yeast, both the nuclear and mitochondrial forms of RNase Z are encoded by a nuclear gene (TRZ1) (15). Again, this enzyme is also implicated in ribosomal RNA maturation. INTRODUCTION. Mitochondria are organelles found in the vast majority of all eukaryal organisms.

They are remnants of an ancient α-proteobacterial endosymbiont, the genome of which has been partly lost or transferred to the nucleus throughout evolution (Gray, Burger and Lang ).Mitochondria consist of two membranes, the inner and outer mitochondrial Cited by: 9.

Mitochondria play a critical role in the generation of metabolic energy in eukaryotic cells. As reviewed in Chapter 2, they are responsible for most of the useful energy derived from the breakdown of carbohydrates and fatty acids, which is converted to ATP by the process of oxidative phosphorylation.

Most mitochondrial. The Biogenesis of Mitochondria: Transcriptional, Translational and Genetic Aspects covers the symposium, ""Biogenesis of Mitochondria"", held in Rosa Marina near Bari, Italy in June Organized into three parts, this book first discusses the mechanism, products, and role of mitochondrial DNA in mitochondrial transcription.

Mitochondria are organelles in eukaryotic cells that play a major role in metabolism, especially the synthesis of ATP. During evolution, mitochondria have lost or transferred most of their genes to the nuclei, significantly reducing the size of their genome ().In yeast, all but one of the few remaining protein genes encode subunits of respiratory chain complexes, whose synthesis.

Disruption of the yeast MTU1 gene eliminated the 2-thio modification of mt tRNAs and impaired mitochondrial protein synthesis, which led to reduced respiratory activity. Furthermore, when MTO1 or MSS1, which are responsible for the C5 substituent of the modified uridine, was disrupted along with MTU1, a much more severe reduction in mitochondrial activity.

Isolation of Yeast Mitochondria and Study of Mitochondrial Protein Translation Johannes M. Herrmann, Heike Fölsch, Walter Neupert, and Rosemary A. Stuart I. Introduction The formation of mitochondria is a detailed process that involves the precise cooperation of two separate genetic systems, one in the mitochondria.

Due to fundamental similarities between the yeast Saccharomyces cerevisiae and multicellular organisms at the molecular level, and the powerful range of experimental tools available for this yeast, S.

cerevisiae is proving an ideal model system for studies on protein synthesis. Structure Determination. Ribosomes purified from yeast mitochondria were contaminated with cytoplasmic ribosomes and free 54S (fig.

S1A). The use of maximum likelihood image classification techniques enabled sorting of these classes of particles into separate groups (fig. S1B).A preliminary reconstruction of the 74S mitochondrial. Highlights Mitochondrial (mt) RNA polymerases (RNAP) serve as an intriguing link between single and multi-subunit RNAPs.

Although structurally similar to T7 RNAP, the mt RNAPs depend on accessory Cited by:   Yeast is one of the oldest domesticated organisms and has both industrial and domestic applications.

In addition, it is very widely used as a eukaryotic model organism in biological research and has offered valuable knowledge of genetics and basic cellular processes. In fact, studies in yeast. Our understanding of protein factors that modulate mitochondrial transcription or couple RNA synthesis with RNA processing and degradation is limited, and is derived primarily from genetic and biochemical studies in the budding yeast.

Due to fundamental similarities between the yeast Saccharomyces cerevisiae and multicellular organisms at the molecular level, and the powerful range of experimental tools available for this yeast, S. cerevisiae is proving an ideal model system for studies on protein synthesis.

The complete model of the yeast mitoribosome contains two rRNA molecules (21S rRNA in the mt-LSU and 15S rRNA in the mt-SSU), 73 proteins (table S2), and a single mt-tRNA bound at the E site (fig.

S4) that is presumably a mixture of the 24 mt-tRNAs encoded by the yeast mitochondrial Cited by: Mitochondria from yeast to humans use a single-polypeptide catalytic RNA polymerase related to enzymes from bacteriophage.

They also require separable transcription factors necessary for initiation at promoter sequences on the mitochondrial Author: Elizabeth A. Amiott, Judith A. Jaehning. The Biogenesis of Mitochondria: Transcriptional, Translational and Genetic Aspects covers the symposium, ""Biogenesis of Mitochondria"", held in Rosa Marina near Bari, Italy in June Organized into three parts, this book first discusses the mechanism, products, and role of mitochondrial DNA in mitochondrial.

Mitochondrial Genomics and Proteomics Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial. The synthesis of cytochrome b in yeast depends on the expression of both mitochondrial and nuclear gene products that act at the level of processing of the pre-mRNA, translation of the mRNA, and.

tRNA import into yeast mitochondria is a mechanism of translational adaptation to stress conditions. At the left side of the scheme, normal conditions of growth (30 °C), when both lysine codons. Disruption of the yeast MTU1 gene eliminated the 2-thio modification of mt tRNAs and impaired mitochondrial protein synthesis, which led to reduced respiratory activity.

Furthermore, when MTO1. Mitochondrial RNA Maturase Gene Transfer e Yeast Nucleus Can Contro itochondrial mRNA Splici ues, A. Dalahodde, and CL Jacq Genetique Moleculaire du C.N.R.S.

Laboratoire propre associe a I’. There is a single gene for the RNA from the large subunit of yeast mitochondrial ribosomes5, but, nevertheless, this RNA hybridises to non-adjacent fragments on the physical map of mtDNA6.

imports the cytosolic pathway for Gln-tRNA synthesis into the mitochondrion Article (PDF Available) in Genes & Development 19(5) April with 35 Reads How we measure 'reads'. Chemically synthesized deoxyribo-icosanucleotides corresponding to sequences 21 to 50 of yeast alanine tRNA as described in Fig.

nona- hepta- and penta-nucleotides (Nona-I, Hepta-I, and. Trans-kingdom rescue of Gln-tRNAGln synthesis in yeast cytoplasm and mitochondria Article (PDF Available) in Nucleic Acids Research 40(18) July with 59 Reads How we measure 'reads'.

Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria.

In the past decade, mitochondrial research has elucidated the important influence of mitochondrial. Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology.

Whether you are an established yeast. Abstract. Mitochondrial promoters of Saccharomyces cerevisiae share a conserved −8 to +1 sequence with +1+2 AA, AG or AT initiation sequence, which dictates the efficiency of transcription initiation by the mitochondrial RNA polymerase Rpo41 and its initiation factor Mtf1.

We used 2-aminopurine fluorescence to monitor promoter melting and measured the k cat /K m of 2-mer synthesis. Studies were undertaken to determine if mitochondrial rRNA synthesis in yeast is regulated by general cellular stringent control mechanism.

Those variables affecting the relaxation of a cycloheximide. Get this from a library. Intersection of RNA Processing and Fatty Acid Synthesis and Attachment in Yeast Mitochondria. [Melissa Schonauer] -- Intersections of distinct biological pathways in cells allow for nodes of metabolic regulation.

This work describes the discovery of the intersection of two pathways in yeast mitochondria: RNA. This article is from Nucleic Acids Research, volume AbstractInitial steps in the synthesis of functional tRNAs require 5′- and 3′-processing of.

Get this from a library. Protein synthesis and targeting in yeast. [Alistair J P Brown; Mick F Tuite; John E G McCarthy; North Atlantic Treaty Organization.

Scientific Affairs Division.;] -- Due to fundamental similarities between the yeast Saccharomyces cerevisiae and multicellular organisms at the molecular level, and the powerful range of experimental tools available for this yeast.

Abstract. The experiments of McLean et al. () first showed definitively the uptake of amino acids and their incorporation into proteins through peptide linkages by mitochondria. The biosynthesis of proteins by mitochondria in vivo and in vitro is indeed a real property of these organelles.

The biosynthesis of proteins in vitro is not due (1) to bacteria contaminating the mitochondrial. Get this from a library! Protein Synthesis and Targeting in Yeast. [Alistair J P Brown; Mick F Tuite; John E G McCarthy] -- Due to fundamental similarities between the yeast Saccharomyces cerevisiae and multicellular organisms at the molecular level, and the powerful range of experimental tools available for this yeast.Ruth Stuckey (Editor) Díaz de la Loz (Editor) Ralf Erik Wellinger (Editor) Universidad de Sevilla – CSIC, Sevilla, Spain Series: Cell Biology Research Progress, DNA and RNA: Properties and .In omega- strains (not containing this intervening sequence) also a longer form of the 21S rRNA could be observed.

These results suggest that isolated yeast mitochondria are capable of carrying out RNA synthesis .

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